Journal: PLoS ONE

Article Title: Peptide Inhibitor of Complement C1 (PIC1) Rapidly Inhibits Complement Activation after Intravascular Injection in Rats

doi: 10.1371/journal.pone.0132446

Figure Lengend Snippet: Hemolytic assays using (A) Wistar rat serum, (B) mouse plasma and (C) cynomolgus monkey serum were performed with increasing amounts PA-dPEG24 dissolved in 100mM Na 2 HPO 4 with 0.9% NaCl buffer for rat serum, 10mM Na 2 HPO 4 with 0.9% NaCl for cynomolgus monkey serum and 0.9% NaCl for mouse plasma. Serum or plasma were incubated with peptide and then added to human AB erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM.

Article Snippet: In a total volume of 375μL, 1x10 9 human AB erythrocytes (non-sensitized) were combined with various concentrations of PA-dPEG24 (dissolved in 0.1M Na 2 HPO 4 , 0.9% NaCl, pH 7.4) and 50μL of Wistar rat serum (Innovative Research, Inc.) for 15min at 37°C.

Techniques: Incubation